653-658 Hu LAJP 2767:Hu
نویسندگان
چکیده
A highly sensitive liquid chromatographic mass spectrometric (LC-MS/MS) method for determination of pantoprazole in rat plasma using omeprazole as the internal standard (IS) was developed. Plasma samples were precipitated by acetonitrile and separated on a Zorbax SB-C18 column with gradient profile at a flow of 0.4 mL/min. Detection was carried out by SIM mode on an ion-trap LC-MS/MS system with an electrospray ionization interface. The lower limit of quantification (LLOQ) was 5 ng/mL. Calibration curve was linear over the range from 5 to 5000 ng/mL. The intraand inter-run relative standard deviations of the assay were less than 7 %. The mean absolute recoveries determined at the concentrations of 25, 400, and 4000 ng/mL were 87.40 ± 4.40 %, 87.77 ± 3.30 %, and 92.78 ± 5.02 %, respectively. The method was applied to the pharmacokinetic of 15 mg/kg of pantoprazole in six rats.
منابع مشابه
Rapid purification of HU protein from Halobacillus karajensis
The histone-like protein HU is the most-abundant DNA-binding protein in bacteria. The HU protein non-specifically binds and bends DNA as a hetero- or homodimer, and can participate in DNA supercoiling and DNA condensation. It also takes part in DNA functions such as replication, recombination, and repair. HU does not recognize any specific sequences but shows a certain degree of specificity to ...
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